Protocols
IHEC provides access to protocols developed and utilized by the national research consortia. The following documents originate from the NIH Roadmap Project.
DNaseI
- Human hematopoietic cells: DNaseI treatment, crosslinking, and preserving cells for RNA
- Nuclei isolation from human tissue using a Dounce homogenizer and subsequent DNaseI treatment and crosslinking
- Nuclei isolation from human tissue using gentleMACS Dissociator and subsequent DNaseI treatment and crosslinking