Protocols and Standards

Assay Standards

The International Human Epigenomics Consortium (IHEC) has established an Assay Standards Working Group to define the assays required for three distinct classes of reference epigenome, and to define standardized protocols and quality control metrics for each assay. The recommendations are intended to provide a framework for the definition of reference epigenomes to be included within the International Human Epigenome Consortium. These recommendations are minimal standards based on current knowledge of the elements contributing to epigenomic regulation in human cells and the current state of epigenomic mapping technologies, and will be reviewed and updated annually.

IHEC Protocols

Protocols

IHEC provides access to protocols developed and utilized by the national research consortia. The following documents originate from the NIH Roadmap Project.

ChIP-Seq

DNaseI

General Protocols

Methylome Analysis

RNA Analysis